Polymorphism within the 5’UTR genomic region of hepatitis G virus (HGV) isolated from beta-thalassemia population in Iraq

Introduction and Aim: Beta-thalassemia patients develop chronic infections due to the hepatitis G virus (HGV), frequent blood transfusions. This study aimed isolate these viruses from beta-thalassemia Iraqi investigate into 5'UTR genomic region of prevalent genotypes in this region. Materials Methods: The included 154 patients. Blood samples were collected each individual participating study. Genomic RNA was isolated subjected cDNA synthesis. 5' untranslated (5' UTR) DNA amplified by polymerase chain reaction using specific HGV primers sent for sequencing. sequences genotyped bioinformatics tools. Results: results showed infection be 18.2% Sequencing alignment several nucleotide variations. A phylogenetic analysis revealed following infect genotype 4(58.3%), 2b (33.3%) 1b (8.3%). Conclusion: Genotyping gene 1b, 4 among Iraq.